一个PCR相关的专业网站，内容很全 3 T; P7 A J' c: q
http://www.pcrlinks.com/
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) J1 ?, T& ^8 _# n% S- y, D( a实验方法与步骤详解 ( z* Y9 e+ v. W) e- u
http://swjsx.ntmc.edu.cn/Protocol/home.htm
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美国AXYgen公司 2 k. w( W6 ]/ m5 U4 ^9 s
http://www.axygen.com/NewFiles/pcr.html 8 C0 Z) K+ g6 a9 l4 \9 Q3 X
8 f( J0 a; u" q A% W) c% ]$ Dhttp://www.alkami.com/ m2 A+ X: B; U
This 158 page PCR manual covers the following topics # V' A6 h1 f* P* l6 t* i4 s
PCR Primer design 5 c9 q. Q/ }$ D
PCR Methods
& E5 I4 _5 h1 w! UPCR Polymerases 0 f" z: o! N' D# U1 \
PCR Variables
3 p: \ Q' K3 `* @9 K2 `1 lPCR Troubleshooting 3 z, x, l8 W3 v5 @* T& I4 n, h
Special PCR Topics ( J7 S: l. U4 Z+ _2 D: K+ j
Appendixes and a comprehensive Index ! F [9 E# E }& i/ v( O$ y
& i: c8 z2 u' \ xBioGuide - PCR
; x5 ~+ w- z; {& o* D4 p: k5 Qhttp://bip.weizmann.ac.il/mb/bioguide/pcr/contents.html / B, A5 K. u# p6 p' j3 u
魏茨曼基因组和生物信息学研究所的一个网页，介绍PCR的初步知识。
8 b5 s, l* S' E0 ?1 r包括： + K' j$ e+ n6 F0 P7 ]% K6 G- I
What is PCR?
- X/ \4 Z- h5 Y/ F& _What are some good reference books for PCR?
3 D0 |- |9 G- s7 ?: m9 NHow should I select a set of primers to use for PCR?
0 G0 b1 c8 }* `; w w; k4 S. GPrograms for designing PCR primers 5 I/ M9 u, w ?) ]7 M7 {9 `
What is "Hot-start" PCR?
# ?" q0 z+ z9 L H' NWhat is AP-PCR or RAPD PCR?
7 [. z) m5 g1 s" B, ]+ I/ zWhat is "Touchdown" PCR? 5 a; A7 e! p% o1 v8 @0 p
Is there a simple method to sequence lambda, M13, or plasmid clones using PCR?* C! e8 p1 W& u0 D4 a9 O! W& Z
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A Look at PCR Cloning Tools
5 P- I; [/ Z+ P( i0 |+ W) [http://www.dxy.cn/bbs/post/view?bid=67&id=151321&sty=1&tpg=63&age=0
! d" g( z: u: w
9 a7 k4 r/ _; M- dPCR常见问题及回答
! _: C0 h: e: B3 uhttp://www.invitrogen.com.cn/techtitle/main/faq_PCR.htm#2 ; ?' M5 b) i; j& a
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PCR和Realtime PCR的原理讲的很透, 一个介绍Realtime PCR的教学网站，里面的powerpo
, \% ]0 B. ]- y* x2 Qint文件非常好
% s' ]# h' X4 G: [( Ehttp://www.med.sc.edu:85/pcr/realtime-home.htm ; O4 Z7 F' V3 |' ?
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SYBR Green PCR 3 O y3 j' d+ ^- a2 E
http://www.dxy.cn/bbs/post/view?bid=67&id=638781&sty=1&tpg=5&ppg=1&age=0#63878
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5 K4 g% T: D% k& w0 w0 U! k3 {1 V+ X, x; s5 e) X
PCR QUICK GUIDE
, z. v/ i: N0 b7 c* G/ U) N; Phttp://www.wzw.tum.de/gene-quantification/quick-guide-pcr.pdf
: K3 o& O/ I: uhttp://www.sfb363.uni-halle.de/mod-path/differential_display/sturtevant00.pdf
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`5 R" I1 {% v7 f" }, G1 Q. lhttp://www.wooster.edu/biology/courses/bio309/default.html
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, @) W1 d1 l" tPCR拼接
& B. r. M; }3 M9 O4 E; {http://www.dxy.cn/bbs/post/view?bid=67&id=305337&sty=1&tpg=47&age=0
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+ k1 Y9 h' q. S5 q) }+ q知道蛋白如何寻找其氨基酸序列
) O% O' I( a0 h8 z- b5 C, y$ ^http://www.dxy.cn/bbs/post/view?bid=67&id=401195&sty=1&tpg=36&age=0
# ~: e5 e6 h2 P; Qhttp://us.expasy.org/cgi-bin/niceprot.pl?P08253
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7 n# ^ d: w/ y& }; b w关于DNA的flash版和文字版 L5 A4 X( B8 q! m2 w' L
http://www.pbs.org/wgbh/nova/genome/dna.html#
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, O7 N$ i0 l9 f5 ]2 |+ J% H; y$ oABI Prism 7000 简要使用说明 5 ^4 ^5 L1 }: R! F% `
http://www.genomultix.com/tech/7000.htm
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好书推荐：Practical Protocols of Gene Engineering
7 Z' y, I5 F* V, d% ~8 Bhttp://www.estarray.org/books/
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http://www.life.uiuc.edu/bio100/lectures/s02lects/18s02biotechclonerecomb.html l* i3 R% C$ L6 M8 A2 ~, f
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Website dealing with cloning and molecular analysis of genes
4 M9 Z3 g6 E4 y9 L& Z1 Shttp://www.ndsu.nodak.edu/instruct/mcclean/plsc431/cloning/clone3.htm
7 Z0 Q, d9 Z7 s- {( u* S5 pSome really wonderful illustrations of the techniques of genetic engineering! : A% }5 ?9 p; m. N) n
; o( r! v& s/ l Z% d: `http://www.uic.edu/classes/phar/phar331/lecture6/ 0 B& ~0 ?, }3 [+ A H w
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, n8 I4 n& m" r7 HA truly amazing global resource page on cloning, stem cell research, and relat
# F( j3 f2 P- o% `* w7 red ethical issues, including recent news articles and legislative updates
: ]' p% O9 x1 N; |& I, j- V: f- Fhttp://genomics.phrma.org/cloning.html 9 i4 V" T5 T3 L8 U
; f& W7 S% ] [- T* @5 R* b4 I$ y$ ? N* P$ Z3 y8 A
Animation and article on interspecies cloning
% l T# ~6 m h$ l! U- zhttp://www.brookscole.com/chemistry_d/templates/student_resources/0030244269_c
( {! u; R9 s) S) R1 Eampbell/HotTopics/InterspeciesCloning.html
+ s( ?! ?5 W$ m% I i9 tFrom PBS...an animation that illustrates how stem cells are cultivated
# `- X" p9 L/ h) ahttp://www.pbs.org/newshour/health/stem_cells.swf ) f- _! E: B% h
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0 c, z% @* y0 qAnimations illustrating the use of viruses as vectors for gene therapy
5 v$ R$ ]& U% a! @; o, O1 R" xhttp://www.technologyreview.com/articles/5940.htm
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C6 Z, v: H- e; vAnimations of techniques used for cloning mammals, as well as a create-your-ow
5 n l5 n! k: [. U& ~& T+ H; A J3 ^3 ]n-clone link
j8 Y* Q7 [/ F$ j- H, ohttp://library.thinkquest.org/24355/data/details/media/index.html
5 ^+ s% B5 `% ~" L8 qAnother animation of PCR
$ k' ~; y9 F/ A. }2 Vhttp://www.people.virginia.edu/~rjh9u/pcranim.html ; V$ ~( Z T }9 M; j% U5 ^3 p
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; K) U# P d* c) BAnimations of restriction digests and gel electrophoresis
! P" Y4 z v( d: Zhttp://www.biology.ucsc.edu/classes/bio20L/animate 4 D; k; I! E" t1 w* g# X" ]
3 O0 v' \- a3 x0 J/ g/ f( IGel electrophoresis techniques and simulations ! D0 T% `0 K, x1 L
A wonderful website that includes links to a virtual lab on agarose gel electr
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" ]8 t8 ~' Q/ ~; Y3 Q4 D2 ~/ Z# Dhttp://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/gels/ - s% R4 \) }+ ~: t* e
A Massachusetts Institute of Technology website dealing with Southerns, Northe! r) p+ D" G: _: X/ B, x* S
rns, Westerns, and cloning:
) P9 `+ e1 b2 g# {http://web.mit.edu/esgbio/www/rdna/rdna.html 8 o3 B& @: r% T- \) J8 G
2-D gel electrophoresis of proteins: " h& i; Q; h9 c2 w- t
http://www.fitzscipress.com/Chap05.pdf : l( j' z/ N+ I0 J
A website containing lots of links to electrophoresis techniques and simulatio
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http://biobenchelper.hypermart.net/pr/electrophoresis.htm 9 t( ^! Q2 Q7 T, m2 }6 v
Want to learn more about chemical bonds? Check out this website: ! Y& l' H; }( u( w' H7 f
http://hyperphysics.phy-astr.gsu.edu/hbase/chemical/bond.html + `% E/ v# A: v% [, X' j# }: U
' S4 `" D6 Q. R: s* ^* b多个录象-多个著名公司 / T0 g- p4 x0 W: T# J
http://www.biocompare.com/video.asp 4 G" a4 u2 r$ r' `% i' R; U0 g+ v
1 k# a0 C- x; |1 ], U* CPCR和 RFLP动画片 * k% F) f+ I9 X C, h' l3 p1 g
http://medtech.cls.msu.edu/ISL/immunology/pcr.htm
, R/ H& `1 k. i2 i0 _' I9 Q& N k& Ahttp://medtech.cls.msu.edu/ISL/immunology/rflp.htm
4 f/ b, z' l% f0 g z# q) u7 G+ K7 w4 i* t$ H: l6 ~: `7 C; t/ v
redasoft visual cloning 2000 patch
% Y) k4 F* m" G) zhttp://www.dxy.cn/bbs/post/view?bid=67&id=496454&sty=1&tpg=26&age=0
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; H8 F, T: k9 R9 R% A* o分子生物学屏幕保护程序下载
2 H: j$ O3 s8 p# }8 e- b4 O% Ppcr $ j0 p. S* v. k A3 v
http://biosoft.biosino.org/down/PCR.exe U! ?* P3 u5 [2 Y
蛋白
1 A4 ~% P- q; `5 y Vhttp://biosoft.biosion.org/down/ssaver.zip . Z- w! g; @$ ~, x$ s! Y3 h" x3 o1 K
amavi100 4 O( {4 p0 S+ K. _" H D
http://ourworld.compuserve.com/homepages/esmsoftware/amavi100.zip
M O6 @! D9 z" o4 p2 Fdna旋转
% S7 v, g* q2 |6 A+ p0 K! {http://www.rocketdownload.com/dnload/dna.exe
& w0 Z8 K5 Z# D C# n. Uddnabase # m$ @, I* @3 u
http://www.rocketdownload.com/dnload/ddnabase.exe 7 n5 H7 f2 e) k5 q- ]' N8 ]
cyclovir
. V# K) n6 L s' ?/ qhttp://mama.indstate.edu/pfaffle/software/cyclovir.exe 3 D; F* {/ L. d5 K
3 e# a9 k8 a( A/ f0 thttp://biosoft.biosino.org/down/source.exe , i/ g2 I0 K! f. n: t4 C
http://ftp.monash.edu.au//pub/win.galttech/gtmolecules.zip |
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