一个PCR相关的专业网站，内容很全
4 I+ f6 O8 j" H+ @http://www.pcrlinks.com/
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' W6 K2 m: P8 p$ Q6 w+ `实验方法与步骤详解
- P: [, p& S' ^http://swjsx.ntmc.edu.cn/Protocol/home.htm / A# p# D1 u0 \9 ]" P. \) P, K
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美国AXYgen公司
* O- p2 K8 Y" O/ j" v- chttp://www.axygen.com/NewFiles/pcr.html
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http://www.alkami.com/
& X4 ^1 H, Y1 D2 A2 tThis 158 page PCR manual covers the following topics
6 C9 Y3 Y5 n f) {PCR Primer design . {! M/ c+ z- d6 U3 w3 }
PCR Methods
% D9 |1 |; P7 SPCR Polymerases $ ~0 C) \6 J# ~
PCR Variables
, v% l: `3 {; M2 {: nPCR Troubleshooting " G4 p% ^: m1 V- I) d
Special PCR Topics # H% K$ G; J9 c, q
Appendixes and a comprehensive Index & I* X3 K' L# o# L0 E5 [
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BioGuide - PCR 9 g' Z, J0 U9 \& y: J
http://bip.weizmann.ac.il/mb/bioguide/pcr/contents.html v1 z( @& F2 H$ M& O7 O
魏茨曼基因组和生物信息学研究所的一个网页，介绍PCR的初步知识。 & b( I# c% ^0 p7 V& T& }- m) N- r
包括： 3 t1 b3 m# @5 p+ F- Z# b( k
What is PCR?
) E$ k4 w7 ~' y, MWhat are some good reference books for PCR?
# o `) s' e3 {How should I select a set of primers to use for PCR?
/ J: J2 W* Q ^Programs for designing PCR primers
: q" M, G7 z5 J" Q7 u8 b- w2 cWhat is "Hot-start" PCR?
8 z Q: ^1 V3 e! o% ^& VWhat is AP-PCR or RAPD PCR?
4 G# p- ]0 L& f. Y9 q* _What is "Touchdown" PCR? $ n1 c, M9 _+ _" D
Is there a simple method to sequence lambda, M13, or plasmid clones using PCR?
8 p& n o( c8 k- }5 K! G1 | i5 C+ k# M1 M4 s; f {: ^
& u# u' e( J6 j3 G% ^A Look at PCR Cloning Tools
0 ?( q3 I. A. x, N, f/ @http://www.dxy.cn/bbs/post/view?bid=67&id=151321&sty=1&tpg=63&age=0
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PCR常见问题及回答
0 U: v4 A' v2 c/ _# u' \http://www.invitrogen.com.cn/techtitle/main/faq_PCR.htm#2
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; C4 j; v) Y; U3 [PCR和Realtime PCR的原理讲的很透, 一个介绍Realtime PCR的教学网站，里面的powerpo9 R( ~* a; u' @ o3 f2 c- I
int文件非常好
* u) w2 {* A5 Phttp://www.med.sc.edu:85/pcr/realtime-home.htm 7 [3 V) F& ^% ?% n5 x. N
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SYBR Green PCR
" t5 Y$ ~9 V3 u0 e$ _http://www.dxy.cn/bbs/post/view?bid=67&id=638781&sty=1&tpg=5&ppg=1&age=0#63878
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% N; G0 D' S/ A _- V: X/ APCR QUICK GUIDE
; N2 X0 M" R1 J# }$ U$ Nhttp://www.wzw.tum.de/gene-quantification/quick-guide-pcr.pdf
0 r2 u1 ~8 G9 R9 k% E0 mhttp://www.sfb363.uni-halle.de/mod-path/differential_display/sturtevant00.pdf & ?- D: V- J) c* M
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http://www.wooster.edu/biology/courses/bio309/default.html ( F! D- Y) `. q; W4 S! _
" _/ H7 [ i0 m) T0 `PCR拼接 9 ^% a y/ U0 i5 i" B% u# U5 ^4 F
http://www.dxy.cn/bbs/post/view?bid=67&id=305337&sty=1&tpg=47&age=0 . Y0 B" @- `# J9 l( P$ n7 O
( S/ p/ I( P' i+ A知道蛋白如何寻找其氨基酸序列 ' R; d5 F/ K. M$ U5 ?( [" H
http://www.dxy.cn/bbs/post/view?bid=67&id=401195&sty=1&tpg=36&age=0
7 G/ \0 h+ x2 a# P: H; Fhttp://us.expasy.org/cgi-bin/niceprot.pl?P08253 5 X% G. Z3 w0 H3 D7 S) G: \+ |
& O6 W8 c4 U2 T! X$ a p. c关于DNA的flash版和文字版 0 C$ v$ w) m, M- k3 s
http://www.pbs.org/wgbh/nova/genome/dna.html#
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ABI Prism 7000 简要使用说明
' O! v# l& `% B0 _) P# ~. Phttp://www.genomultix.com/tech/7000.htm $ j# P; g0 f' S
* K% S) o- W* Y! t好书推荐：Practical Protocols of Gene Engineering
- c# {, ~- O# Bhttp://www.estarray.org/books/
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# G4 T# {4 _& a6 i8 v$ M, bhttp://www.life.uiuc.edu/bio100/lectures/s02lects/18s02biotechclonerecomb.html
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3 b; R8 W$ G+ N. B B ~Website dealing with cloning and molecular analysis of genes 7 n7 z* _" f! {* R6 r) G
http://www.ndsu.nodak.edu/instruct/mcclean/plsc431/cloning/clone3.htm & Y8 V" q8 H0 `" _% O# i( p& F& c
Some really wonderful illustrations of the techniques of genetic engineering! & M( o& n5 J8 G% A* d$ p! P* N
: c5 F& G' X3 v% D$ g* }- k6 ehttp://www.uic.edu/classes/phar/phar331/lecture6/ 3 R$ ]& w* [) F' d3 y$ T, x) I
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/ N6 A! ~1 S8 \5 D/ G5 PA truly amazing global resource page on cloning, stem cell research, and relat
& R8 ?& X* ?+ O6 b" hed ethical issues, including recent news articles and legislative updates
& T% X6 Y( o$ y5 k0 ~- shttp://genomics.phrma.org/cloning.html " ^, V9 S9 `6 ~2 Q" U* M
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4 N1 ]& d0 A/ rAnimation and article on interspecies cloning
- y$ K+ i( r1 G! n( G$ _5 z# D: {/ Nhttp://www.brookscole.com/chemistry_d/templates/student_resources/0030244269_c7 [. ^7 W- S7 k0 m/ n" j8 ]
ampbell/HotTopics/InterspeciesCloning.html : s' {7 t1 K, H2 N7 U
From PBS...an animation that illustrates how stem cells are cultivated + R1 { D4 p3 G( B6 T' `4 }$ z
http://www.pbs.org/newshour/health/stem_cells.swf
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Animations illustrating the use of viruses as vectors for gene therapy . G) ?- y0 [4 f- F2 u8 ~* A+ c; q, s
http://www.technologyreview.com/articles/5940.htm ' z9 U0 {1 C! u
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7 b% \7 w2 S( H1 nAnimations of techniques used for cloning mammals, as well as a create-your-ow8 X1 F4 `6 S# j: z0 X/ R. \; T
n-clone link ! [9 o( V- w; G" ]6 u" N
http://library.thinkquest.org/24355/data/details/media/index.html 5 j' Y5 z% ^: ^; g9 g
Another animation of PCR
" D( Y2 b( @/ whttp://www.people.virginia.edu/~rjh9u/pcranim.html $ h1 a" r5 N8 q/ n( N& c" C& k& Q* f
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) p7 e- r( J# CAnimations of restriction digests and gel electrophoresis
) I* }4 g# k) whttp://www.biology.ucsc.edu/classes/bio20L/animate
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Gel electrophoresis techniques and simulations / t8 Q4 V" s& p" Z+ N0 ~
A wonderful website that includes links to a virtual lab on agarose gel electr. n, v+ u* [5 n/ ?. d \( _8 n+ z
ophoresis:
* ]2 k# l1 q$ g1 S9 y+ W$ i, \$ Z4 Chttp://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/gels/ 8 Y$ N, V- G2 }" F( L
A Massachusetts Institute of Technology website dealing with Southerns, Northe5 [$ o5 [) D- Y, O# _- m4 [
rns, Westerns, and cloning:
# F) W$ d1 ]" B, lhttp://web.mit.edu/esgbio/www/rdna/rdna.html
5 G$ {! z |7 w" y2-D gel electrophoresis of proteins:
" Q$ x, b$ o( M+ o+ \http://www.fitzscipress.com/Chap05.pdf 7 \6 L/ j# h6 z* j; c
A website containing lots of links to electrophoresis techniques and simulatio
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http://biobenchelper.hypermart.net/pr/electrophoresis.htm
9 W1 v F9 R/ l+ U2 e" j* HWant to learn more about chemical bonds? Check out this website:
/ _5 l/ w6 K+ `6 ehttp://hyperphysics.phy-astr.gsu.edu/hbase/chemical/bond.html
# p6 G/ k9 C2 ]9 j( Z& W8 j3 e2 [. z/ `2 H( T8 [+ Y
多个录象-多个著名公司
% B3 e, `7 m/ w M* @5 m# Yhttp://www.biocompare.com/video.asp
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PCR和 RFLP动画片 ^# L6 p; D t7 C( W2 {
http://medtech.cls.msu.edu/ISL/immunology/pcr.htm
4 ?& I" S! o6 a8 thttp://medtech.cls.msu.edu/ISL/immunology/rflp.htm 6 L' ~/ y! T1 ^! r- ]* _* ~
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redasoft visual cloning 2000 patch 1 Y) l1 ]# R0 z4 B
http://www.dxy.cn/bbs/post/view?bid=67&id=496454&sty=1&tpg=26&age=0 4 \% l6 c' ?) ?2 K
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分子生物学屏幕保护程序下载
$ T' g- _6 x9 T/ }; G5 hpcr
8 K+ n6 E/ n i4 n- C1 r* T$ {http://biosoft.biosino.org/down/PCR.exe ' e$ G8 R J; s9 W
蛋白 * ?6 Y/ \+ T7 g0 O# p
http://biosoft.biosion.org/down/ssaver.zip ' y3 U$ j; w( y+ R* U. [/ y% b
amavi100 ! B5 C* Z2 Q5 v& e. U
http://ourworld.compuserve.com/homepages/esmsoftware/amavi100.zip
) a& L9 i& _- `dna旋转 4 S. C* w" E+ D% S- d
http://www.rocketdownload.com/dnload/dna.exe 3 T; T) m" {5 E' V
ddnabase
# Q: b0 g; R6 x& `( ~http://www.rocketdownload.com/dnload/ddnabase.exe
7 A1 @* A: \8 Y1 B7 p; ]' Scyclovir
/ D- K; |0 `, ~: x8 ghttp://mama.indstate.edu/pfaffle/software/cyclovir.exe 0 L F) ?% Z$ ^- U; P; a! U
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http://biosoft.biosino.org/down/source.exe
^' B, q( T/ f$ n! T R0 Y; H3 e* {6 Ehttp://ftp.monash.edu.au//pub/win.galttech/gtmolecules.zip |
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