一个PCR相关的专业网站，内容很全 * s% r$ w1 }" p4 C- H
http://www.pcrlinks.com/ 0 L9 Q1 M* O& Z- O% a! n1 O1 w
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实验方法与步骤详解
& m# n8 d0 r) ]: ~ Y/ ?5 V8 b% uhttp://swjsx.ntmc.edu.cn/Protocol/home.htm 5 z" ]* G# z8 |2 w
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美国AXYgen公司 - B! r9 c9 Y. D& w9 y9 t9 J
http://www.axygen.com/NewFiles/pcr.html , N: q8 S# i) t
O5 v- n- }! h. S- c' Khttp://www.alkami.com/
- \ l# `' [. d* }( e8 oThis 158 page PCR manual covers the following topics
2 F8 w: E; l, fPCR Primer design # c; b i2 w! w+ \! ^9 U% H
PCR Methods 8 P+ x! T& ]; n" B& I
PCR Polymerases , W' w+ r% t, B! S3 ~
PCR Variables % z" r1 e* `+ N, L1 h l
PCR Troubleshooting 3 t/ R. G. s$ m
Special PCR Topics
* Q/ r2 J/ Y! {$ i" sAppendixes and a comprehensive Index
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BioGuide - PCR `! F( E- v$ T- S0 L
http://bip.weizmann.ac.il/mb/bioguide/pcr/contents.html 3 u }2 F% D; V2 _7 _4 @: B
魏茨曼基因组和生物信息学研究所的一个网页，介绍PCR的初步知识。 , N" ~/ o$ o7 ~$ {; l8 P. X" B* x
包括： ; i# q3 L) C+ ?7 P: Z3 N0 a
What is PCR?
/ N# v8 X! U0 _6 r M0 LWhat are some good reference books for PCR? * z; n Z; u( t. U; ~6 U# o
How should I select a set of primers to use for PCR? 5 L, a$ i# G3 I3 D. s$ _3 S
Programs for designing PCR primers - d# t% X" v: ~- ?* e5 i
What is "Hot-start" PCR?
" m" S7 M6 I0 o" \0 BWhat is AP-PCR or RAPD PCR?
" a) g1 a P5 Q2 m% j( uWhat is "Touchdown" PCR? - L/ N0 s- G# [5 L
Is there a simple method to sequence lambda, M13, or plasmid clones using PCR?5 N5 q+ ?4 C$ m; X9 X: O0 m
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A Look at PCR Cloning Tools 6 x6 F& j+ C8 k: f
http://www.dxy.cn/bbs/post/view?bid=67&id=151321&sty=1&tpg=63&age=0 % _0 w( {9 Y9 ^3 {+ o& b' @' K) i) n
* X- f6 [; ~' }5 e1 v, @! a9 t% \
PCR常见问题及回答
. V- j s$ V. K- t# ]http://www.invitrogen.com.cn/techtitle/main/faq_PCR.htm#2 : a$ j" Q( }: s/ l& G# o6 Y" g
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PCR和Realtime PCR的原理讲的很透, 一个介绍Realtime PCR的教学网站，里面的powerpo
/ d8 t Y, j% E$ N3 d& X" Uint文件非常好 7 z. a, J8 X; E+ z, v' j
http://www.med.sc.edu:85/pcr/realtime-home.htm
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3 Y3 s) c. z+ k' n, }- U2 {SYBR Green PCR
- y& S9 P; R4 Y, Thttp://www.dxy.cn/bbs/post/view?bid=67&id=638781&sty=1&tpg=5&ppg=1&age=0#63878
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PCR QUICK GUIDE $ H, z) D& g/ L- B3 P: Z
http://www.wzw.tum.de/gene-quantification/quick-guide-pcr.pdf
- ~/ \- l+ U0 K0 E5 thttp://www.sfb363.uni-halle.de/mod-path/differential_display/sturtevant00.pdf
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http://www.wooster.edu/biology/courses/bio309/default.html + Y. V6 C9 [1 q" S& D, a0 _
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PCR拼接
e; R8 G; B5 U* h; I8 ~http://www.dxy.cn/bbs/post/view?bid=67&id=305337&sty=1&tpg=47&age=0 6 @8 H' I) C. R$ R
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知道蛋白如何寻找其氨基酸序列
% w$ c" n- X% k: p) Y: ?8 ~http://www.dxy.cn/bbs/post/view?bid=67&id=401195&sty=1&tpg=36&age=0
) K+ a- [8 ^9 V/ {http://us.expasy.org/cgi-bin/niceprot.pl?P08253
9 @2 X* X ?3 ^& }1 x4 W- S8 H! i3 C9 l* s& u
关于DNA的flash版和文字版
5 |$ W5 H3 W. ^) ?& e0 whttp://www.pbs.org/wgbh/nova/genome/dna.html#
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2 u* z. W5 j+ J7 n% `0 QABI Prism 7000 简要使用说明 5 T0 |& P6 c* k/ V# Q ~, f4 y$ A
http://www.genomultix.com/tech/7000.htm * z& }" x/ [; q8 \* B8 Q4 Q
, p8 M4 u; X. c0 o% I; _好书推荐：Practical Protocols of Gene Engineering 5 d$ Z8 y3 l7 n6 r8 W9 s9 x. t
http://www.estarray.org/books/ 6 G9 _* X. x( p) w
+ {, v, q% `( |( \http://www.life.uiuc.edu/bio100/lectures/s02lects/18s02biotechclonerecomb.html
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* i# H1 ^4 b* v+ MWebsite dealing with cloning and molecular analysis of genes
5 e* R& j: P0 a% Whttp://www.ndsu.nodak.edu/instruct/mcclean/plsc431/cloning/clone3.htm " y+ H* @2 n3 z6 J
Some really wonderful illustrations of the techniques of genetic engineering! ' t' T4 r7 [" z2 W
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http://www.uic.edu/classes/phar/phar331/lecture6/ ! _0 g+ h# v2 ?: M
# d! g, @5 a7 P) T$ U3 b) [
* L1 y3 P7 G# p, I8 EA truly amazing global resource page on cloning, stem cell research, and relat
% h5 {- m2 N, z( r* Ied ethical issues, including recent news articles and legislative updates
1 {# g4 s4 a) b# Z" [' Ghttp://genomics.phrma.org/cloning.html ( f" f+ G: T7 Z# [
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# E& s. W! {' d% N, k9 AAnimation and article on interspecies cloning
/ l0 S& |: p8 k( U* j, E: |1 thttp://www.brookscole.com/chemistry_d/templates/student_resources/0030244269_c* Z3 \9 i4 C) V% R& |! {7 G! B
ampbell/HotTopics/InterspeciesCloning.html
9 J5 E+ x h; Y: B4 tFrom PBS...an animation that illustrates how stem cells are cultivated
" \7 H# Z& A0 @4 {( ^http://www.pbs.org/newshour/health/stem_cells.swf
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Animations illustrating the use of viruses as vectors for gene therapy
( m7 I$ { e8 q) Rhttp://www.technologyreview.com/articles/5940.htm
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9 D; h& q9 P$ MAnimations of techniques used for cloning mammals, as well as a create-your-ow
2 m' |; L' R) m- ln-clone link
: N, |4 W( I- S" q u) Q. Z; fhttp://library.thinkquest.org/24355/data/details/media/index.html
: h+ o& _1 H2 O/ q0 V$ A+ P" LAnother animation of PCR
( q! {- Q. @9 _9 Whttp://www.people.virginia.edu/~rjh9u/pcranim.html - Z8 p- D9 @, c$ r: V; j
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! C7 b$ w5 D8 `- P8 D7 |$ {% IAnimations of restriction digests and gel electrophoresis ! ]: h7 O1 G4 C8 B% Y4 `
http://www.biology.ucsc.edu/classes/bio20L/animate
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/ f+ R. v) Z$ a$ L% ZGel electrophoresis techniques and simulations
& a6 h2 d Q2 T w' }A wonderful website that includes links to a virtual lab on agarose gel electr
) H7 u3 P# `. b3 Q2 O' tophoresis: - t2 m8 d3 C+ P1 F. {8 E# ?0 F; m E
http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/gels/ ' |. A! S1 r( x! Z0 O- w. x9 v* g( w
A Massachusetts Institute of Technology website dealing with Southerns, Northe9 ]' c9 G" X6 W$ ~. x7 Y
rns, Westerns, and cloning:
" i; ~$ i1 U) |% c' c' jhttp://web.mit.edu/esgbio/www/rdna/rdna.html
- Q2 L& V9 @0 T' a2-D gel electrophoresis of proteins:
% d0 o7 V2 D+ R& e; Xhttp://www.fitzscipress.com/Chap05.pdf ' t" \0 k8 z( x+ y8 T! s k2 D) T
A website containing lots of links to electrophoresis techniques and simulatio
& `# |5 K* R$ t5 p. g" ons:
& |6 G, @, K; l$ m* P' b5 Mhttp://biobenchelper.hypermart.net/pr/electrophoresis.htm 1 ?, b( F2 n" p8 t: a6 ?2 B
Want to learn more about chemical bonds? Check out this website:
( P! k3 ^, m2 j7 \# E! L0 ?http://hyperphysics.phy-astr.gsu.edu/hbase/chemical/bond.html
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多个录象-多个著名公司 8 K0 P" R! F8 M7 O! ^4 p
http://www.biocompare.com/video.asp
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PCR和 RFLP动画片
8 s% a1 H$ i5 Z! a! s1 e8 @http://medtech.cls.msu.edu/ISL/immunology/pcr.htm 6 Z3 D( t1 C6 a% F; i2 l+ E
http://medtech.cls.msu.edu/ISL/immunology/rflp.htm
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redasoft visual cloning 2000 patch . y5 X2 o# F0 T
http://www.dxy.cn/bbs/post/view?bid=67&id=496454&sty=1&tpg=26&age=0 6 G( D. l0 { C' T8 o
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分子生物学屏幕保护程序下载
# e) e1 M7 G7 E$ k9 wpcr , ~) X9 K: O& F4 F6 [$ N8 R
http://biosoft.biosino.org/down/PCR.exe : ?! D/ _3 x8 n- s
蛋白
9 H. \: T" J8 s# v, s1 nhttp://biosoft.biosion.org/down/ssaver.zip & e' T: ?% p0 F. l/ b) G4 d
amavi100 ; q8 B' \' z1 s+ d( A) p
http://ourworld.compuserve.com/homepages/esmsoftware/amavi100.zip 3 |. T9 Y( u- }% V
dna旋转
T) M* A4 @; i( s, i# o b. zhttp://www.rocketdownload.com/dnload/dna.exe
: w0 b* {7 L# n2 Cddnabase
3 ?* D) I J8 t9 P9 q; }http://www.rocketdownload.com/dnload/ddnabase.exe : \' |$ k# K% j- G- W
cyclovir
) X% ^# D. {. r& ^2 w. @http://mama.indstate.edu/pfaffle/software/cyclovir.exe
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, k# ^/ `) j' b: V1 D+ e, p: thttp://biosoft.biosino.org/down/source.exe
A( n F! J, l5 ?5 Z) R9 qhttp://ftp.monash.edu.au//pub/win.galttech/gtmolecules.zip |
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