一个PCR相关的专业网站，内容很全
8 {; R% _8 Z' ~http://www.pcrlinks.com/ / |( T8 h- ^) k) P
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实验方法与步骤详解 1 P- ^9 n; t' H& ~1 f( k9 O+ i! {
http://swjsx.ntmc.edu.cn/Protocol/home.htm
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" j8 {! o) R$ p0 j* A美国AXYgen公司
: w4 `7 L' |& m7 P1 i; h/ Yhttp://www.axygen.com/NewFiles/pcr.html
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t7 N4 z6 E7 \http://www.alkami.com/
: c: K8 C* x" Q( x3 o* R+ X6 oThis 158 page PCR manual covers the following topics
! ^ |" ?2 C' r. nPCR Primer design
5 z2 l4 a* T8 u& Y5 c3 mPCR Methods
/ J* b6 ]7 u" T# P6 gPCR Polymerases
# F, X+ {2 B. \ ]7 i# {PCR Variables 3 a5 Q' l3 ]9 q! c3 j* \' z
PCR Troubleshooting 8 @& ]+ ^/ t" K7 U
Special PCR Topics
+ o; p% A7 E5 k: i# h3 ^Appendixes and a comprehensive Index
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BioGuide - PCR # E0 D/ S2 S- f2 `% \" G
http://bip.weizmann.ac.il/mb/bioguide/pcr/contents.html : o& b2 K5 l- {! B
魏茨曼基因组和生物信息学研究所的一个网页，介绍PCR的初步知识。 # e$ V- A5 n. e2 S' _
包括：
0 d; @7 M! l: W6 ~What is PCR?
( E) c- v n& i$ }What are some good reference books for PCR? ; l0 o2 e! P# g, w
How should I select a set of primers to use for PCR?
" v! D% c6 S! u2 Z. |9 U) X; lPrograms for designing PCR primers
5 c! t; P) W1 t0 I0 @- S( LWhat is "Hot-start" PCR? 9 \# Y) ^! |6 h% J7 M
What is AP-PCR or RAPD PCR?
6 m* u' p8 K, N6 c0 _0 x8 lWhat is "Touchdown" PCR? 7 n9 a6 N) ~9 h
Is there a simple method to sequence lambda, M13, or plasmid clones using PCR?
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* ^" i ]" K9 Y: WA Look at PCR Cloning Tools : o2 C1 o4 G; h" ?
http://www.dxy.cn/bbs/post/view?bid=67&id=151321&sty=1&tpg=63&age=0
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* I( ` [7 x4 ^PCR常见问题及回答
" K( M! s* o J: Khttp://www.invitrogen.com.cn/techtitle/main/faq_PCR.htm#2 $ o% y0 B. [2 h) `
0 U. V& l( t3 j6 Y( ~0 cPCR和Realtime PCR的原理讲的很透, 一个介绍Realtime PCR的教学网站，里面的powerpo
C; d( O; P8 zint文件非常好
( y- Z1 t+ w8 s1 f( V1 Dhttp://www.med.sc.edu:85/pcr/realtime-home.htm : O& y4 `5 J3 ?9 X
' C: n, U, E6 m' s8 d8 j3 Q# |: B' j, ASYBR Green PCR
7 O0 R. {1 J8 N4 q3 I) W' ihttp://www.dxy.cn/bbs/post/view?bid=67&id=638781&sty=1&tpg=5&ppg=1&age=0#638785 g8 j* Y$ `, x+ Y% r0 V4 W6 z/ l, T
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PCR QUICK GUIDE 0 U; F; [) `9 h6 c( A4 ?
http://www.wzw.tum.de/gene-quantification/quick-guide-pcr.pdf 1 G3 f b, Z% `1 L2 P7 e" l
http://www.sfb363.uni-halle.de/mod-path/differential_display/sturtevant00.pdf
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http://www.wooster.edu/biology/courses/bio309/default.html 7 Q5 G1 C4 t! J2 M3 H. `! s5 H
4 Y" |: N4 K) z; NPCR拼接 9 C# Q4 K% g" D2 c
http://www.dxy.cn/bbs/post/view?bid=67&id=305337&sty=1&tpg=47&age=0
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& v7 B) @1 f8 M知道蛋白如何寻找其氨基酸序列 % n( o1 j( n# G; W$ o
http://www.dxy.cn/bbs/post/view?bid=67&id=401195&sty=1&tpg=36&age=0
1 Q3 [7 A7 F( K& S7 uhttp://us.expasy.org/cgi-bin/niceprot.pl?P08253 & w& g8 J3 |% @8 g% ?" d
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关于DNA的flash版和文字版
5 B9 j9 O u9 xhttp://www.pbs.org/wgbh/nova/genome/dna.html#
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6 c! p. u, q$ E3 J+ _ABI Prism 7000 简要使用说明
- u8 n, E8 j fhttp://www.genomultix.com/tech/7000.htm
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2 F x5 M0 q9 q- p- J; e/ C+ w好书推荐：Practical Protocols of Gene Engineering
3 ]; \& _4 m; j! [; S shttp://www.estarray.org/books/
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5 T: k* o! y, shttp://www.life.uiuc.edu/bio100/lectures/s02lects/18s02biotechclonerecomb.html
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Website dealing with cloning and molecular analysis of genes
" `0 \- q& ~- h- O/ `http://www.ndsu.nodak.edu/instruct/mcclean/plsc431/cloning/clone3.htm " Y- J( }( a2 \+ n3 @5 P! ]
Some really wonderful illustrations of the techniques of genetic engineering! , M( m4 c/ D6 p. c/ g5 d8 X
& t4 B# E% f5 Q% t9 C8 K4 ^- S7 rhttp://www.uic.edu/classes/phar/phar331/lecture6/ 3 s3 I1 `! ^/ S) [
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A truly amazing global resource page on cloning, stem cell research, and relat
0 |( t( r2 F9 L, Ked ethical issues, including recent news articles and legislative updates
4 i4 F* K D, z" O. M: Q2 ]: ~( lhttp://genomics.phrma.org/cloning.html $ c. e2 f. ^/ e z- s5 w
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. l; @' g& h0 ^" i3 F6 ^& dAnimation and article on interspecies cloning
3 ~# t! y O4 e; R2 G- xhttp://www.brookscole.com/chemistry_d/templates/student_resources/0030244269_c; ]8 m" h1 h9 ~- K
ampbell/HotTopics/InterspeciesCloning.html
* x; I* E: M; r* ?+ x7 q0 ?From PBS...an animation that illustrates how stem cells are cultivated
2 y" _' W, m6 ~* R0 e$ Nhttp://www.pbs.org/newshour/health/stem_cells.swf
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Animations illustrating the use of viruses as vectors for gene therapy
' E+ B; V6 C5 c# Y( f* k Vhttp://www.technologyreview.com/articles/5940.htm / b5 R1 D d: }6 z- f
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Animations of techniques used for cloning mammals, as well as a create-your-ow
2 i; C: _) o2 v# ~n-clone link 9 `$ H, Z# G+ G8 m* w0 s9 e5 i: L& l
http://library.thinkquest.org/24355/data/details/media/index.html
% L8 U+ ?& T3 oAnother animation of PCR
0 e$ X; Y; Y4 [ o- Whttp://www.people.virginia.edu/~rjh9u/pcranim.html * ]) J8 o% B' l* n# ~# g
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Animations of restriction digests and gel electrophoresis
5 `: S, E( W5 N2 e) K# n8 Whttp://www.biology.ucsc.edu/classes/bio20L/animate * |+ u) L3 B; @: ?
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Gel electrophoresis techniques and simulations 9 K+ E* X4 R# b& V9 L, Z
A wonderful website that includes links to a virtual lab on agarose gel electr3 F5 }0 N: X9 e. g7 Y/ U! B b
ophoresis:
! }/ N- |" R% @$ P; n3 shttp://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/gels/
' v) e, ^% }/ mA Massachusetts Institute of Technology website dealing with Southerns, Northe7 }+ B* t! R3 x
rns, Westerns, and cloning: ! m! _& c" N1 [" F$ Y0 i
http://web.mit.edu/esgbio/www/rdna/rdna.html
/ I; F O. z- F6 |2-D gel electrophoresis of proteins: C: E$ |/ B9 Y
http://www.fitzscipress.com/Chap05.pdf + O2 M* j; I& j% N# x
A website containing lots of links to electrophoresis techniques and simulatio
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http://biobenchelper.hypermart.net/pr/electrophoresis.htm
8 f S" F4 F9 J# s- r/ fWant to learn more about chemical bonds? Check out this website:
! K# t( H+ y+ `2 l4 l; j, d, _http://hyperphysics.phy-astr.gsu.edu/hbase/chemical/bond.html
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. A9 h9 Q- `# w& ~多个录象-多个著名公司 ( T$ ]& G/ q: k* R
http://www.biocompare.com/video.asp
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$ ~; J/ r$ O" o( L, [8 k0 jPCR和 RFLP动画片 ) m5 g+ P4 z+ c& J% k, _
http://medtech.cls.msu.edu/ISL/immunology/pcr.htm : A+ N6 v+ r" b9 ?9 N% g
http://medtech.cls.msu.edu/ISL/immunology/rflp.htm ! d5 ?- e4 |; O1 H$ K2 H; ]% Y
4 K8 B, q' W( d2 j: I( i" g. k7 Rredasoft visual cloning 2000 patch ; E9 l n: s+ ~# ? y7 R: i
http://www.dxy.cn/bbs/post/view?bid=67&id=496454&sty=1&tpg=26&age=0
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分子生物学屏幕保护程序下载 8 Q% J' x5 N% U% G
pcr
/ m8 n2 ? h7 [' }. `# j$ ^5 s/ fhttp://biosoft.biosino.org/down/PCR.exe
! Y$ w% o$ t5 @* N/ b" N( t; L蛋白
. O$ \! h+ s: K' f3 F& Shttp://biosoft.biosion.org/down/ssaver.zip
5 s$ Q* l9 P& ~& v8 x% d( Oamavi100
: J( n# O% O8 V8 C+ \: U- phttp://ourworld.compuserve.com/homepages/esmsoftware/amavi100.zip
. S, T/ T8 M/ Zdna旋转
& `' m4 w* H& Qhttp://www.rocketdownload.com/dnload/dna.exe - ?8 I+ A) x- B, ?$ h
ddnabase * F- r7 H; r9 k: U+ [1 g
http://www.rocketdownload.com/dnload/ddnabase.exe
s1 W3 C, o' k* D. W Bcyclovir
/ m( I5 e7 a' k. I) ^3 thttp://mama.indstate.edu/pfaffle/software/cyclovir.exe 6 ?8 z9 T& }+ w' t: k# h: Y$ m
# u2 P# o$ U6 F7 E! P; d3 @- E& lhttp://biosoft.biosino.org/down/source.exe
1 u% u; {0 q( o/ [" W4 O8 R4 shttp://ftp.monash.edu.au//pub/win.galttech/gtmolecules.zip |
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