一个PCR相关的专业网站，内容很全
, V! r4 _9 P! f" k4 G+ f# ]3 |http://www.pcrlinks.com/
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+ M8 Q& ^, I! d4 b实验方法与步骤详解 8 d- }( A( n4 Y* _, d3 R, F
http://swjsx.ntmc.edu.cn/Protocol/home.htm
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美国AXYgen公司
% E: f5 T! J( V2 ^http://www.axygen.com/NewFiles/pcr.html 0 v& {0 u& A: L t* ^9 J S
. f: m4 w. K6 l+ m$ T/ `3 k1 r$ Rhttp://www.alkami.com/ 6 X5 w+ u, [) d) P+ ^
This 158 page PCR manual covers the following topics
; {' k& }" Z2 B- N$ b. rPCR Primer design 4 H' y, f+ d4 V3 H" Z! E
PCR Methods
1 F$ ]$ P" l9 j5 ~PCR Polymerases
: O0 v0 }! @5 |# l) NPCR Variables
) t4 ~& r; ]* H: _0 fPCR Troubleshooting
9 z0 B9 c( [8 I6 ?6 g' fSpecial PCR Topics
3 n) D ?# W JAppendixes and a comprehensive Index
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4 d3 v* [$ _8 e8 v( b- [& j# X3 eBioGuide - PCR
( Z* w- y R: V& d. ?( G) s9 thttp://bip.weizmann.ac.il/mb/bioguide/pcr/contents.html ) t$ j3 I& y: Z+ m
魏茨曼基因组和生物信息学研究所的一个网页，介绍PCR的初步知识。
) h, F2 c6 O1 Z& j/ K包括：
% V- K) }" D. [: ~9 C5 I& A6 lWhat is PCR?
) G* R3 r$ f# @5 N) g# MWhat are some good reference books for PCR?
9 g) B `3 r7 hHow should I select a set of primers to use for PCR?
+ s H6 }$ I+ [0 JPrograms for designing PCR primers - g, [$ a4 V' a6 u: e
What is "Hot-start" PCR? : S, V9 k( W& r1 c
What is AP-PCR or RAPD PCR? , \- x; Z: r9 t2 m. o
What is "Touchdown" PCR?
P3 P: U4 S, |* x% L8 i) dIs there a simple method to sequence lambda, M13, or plasmid clones using PCR?
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' }& r6 P6 i! _, q2 K3 zA Look at PCR Cloning Tools % n: S) L6 `1 |! e# r
http://www.dxy.cn/bbs/post/view?bid=67&id=151321&sty=1&tpg=63&age=0
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6 H; n5 c0 _1 X- p* J( ZPCR常见问题及回答 t6 U5 i* [ x+ `# C! z3 R
http://www.invitrogen.com.cn/techtitle/main/faq_PCR.htm#2
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PCR和Realtime PCR的原理讲的很透, 一个介绍Realtime PCR的教学网站，里面的powerpo# [' }9 L5 z- `" T0 Y) X+ |
int文件非常好
4 |. X4 `$ j, f. \4 \$ Zhttp://www.med.sc.edu:85/pcr/realtime-home.htm : q3 \6 J0 z! @0 t2 a; w% [1 [
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SYBR Green PCR
/ t R- s" m7 b1 [# Khttp://www.dxy.cn/bbs/post/view?bid=67&id=638781&sty=1&tpg=5&ppg=1&age=0#638781 D: p, z/ @$ |8 K; b; l
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; b" X5 H6 @9 r- _: wPCR QUICK GUIDE ) t. N0 |. a1 ~. A2 q' v7 }
http://www.wzw.tum.de/gene-quantification/quick-guide-pcr.pdf ) D% `$ c0 Z: ^7 v- z! `
http://www.sfb363.uni-halle.de/mod-path/differential_display/sturtevant00.pdf
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http://www.wooster.edu/biology/courses/bio309/default.html , B/ `, x% [# c5 Y% s0 ?9 i
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PCR拼接
, O; j7 h% J5 }& r' r. ~, U- x! y2 @' Ghttp://www.dxy.cn/bbs/post/view?bid=67&id=305337&sty=1&tpg=47&age=0
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知道蛋白如何寻找其氨基酸序列 # D; _7 V: N( a5 V1 w
http://www.dxy.cn/bbs/post/view?bid=67&id=401195&sty=1&tpg=36&age=0
8 Z& @2 T! ?- H$ \% \) Y: y3 fhttp://us.expasy.org/cgi-bin/niceprot.pl?P08253 7 F" y! l/ n4 z
6 D$ Q+ j0 w) |8 |% z2 b关于DNA的flash版和文字版
* F# h! H, ]. `/ @, C5 }7 s3 B* \http://www.pbs.org/wgbh/nova/genome/dna.html# " s8 y$ e* C" y, R4 m
3 c1 R# u( y/ z; K( _8 OABI Prism 7000 简要使用说明 ) l, X$ o8 P, X$ f6 p# s/ E
http://www.genomultix.com/tech/7000.htm
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0 w) w3 U0 H0 b/ h0 \1 o- [$ D7 f好书推荐：Practical Protocols of Gene Engineering 9 ]1 C9 E8 _* v3 G1 T
http://www.estarray.org/books/
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http://www.life.uiuc.edu/bio100/lectures/s02lects/18s02biotechclonerecomb.html
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Website dealing with cloning and molecular analysis of genes 6 q, L2 U" i+ _
http://www.ndsu.nodak.edu/instruct/mcclean/plsc431/cloning/clone3.htm
0 m3 \+ Y5 r0 E5 pSome really wonderful illustrations of the techniques of genetic engineering!
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http://www.uic.edu/classes/phar/phar331/lecture6/
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A truly amazing global resource page on cloning, stem cell research, and relat& p; a# d2 O" l5 o1 g1 I
ed ethical issues, including recent news articles and legislative updates ; |1 i' k* P( _! ^
http://genomics.phrma.org/cloning.html
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- i6 H0 `" }; o2 qAnimation and article on interspecies cloning * X: `' N2 `: @8 r
http://www.brookscole.com/chemistry_d/templates/student_resources/0030244269_c9 a, t/ ^- _9 z( \4 P8 Z$ ~
ampbell/HotTopics/InterspeciesCloning.html
" d$ Q, I3 ]# I8 m$ G5 c- \From PBS...an animation that illustrates how stem cells are cultivated
6 j0 ]$ \9 P% Y+ U' yhttp://www.pbs.org/newshour/health/stem_cells.swf
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Animations illustrating the use of viruses as vectors for gene therapy
% U; y4 x3 U2 r* I% u9 ]' Yhttp://www.technologyreview.com/articles/5940.htm
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$ s) G6 D; G, R: d0 I, qAnimations of techniques used for cloning mammals, as well as a create-your-ow) t9 i( V; Q# {1 b2 M" v
n-clone link : U/ `0 R4 y& M
http://library.thinkquest.org/24355/data/details/media/index.html + y6 r; z& Z$ [/ U3 |: Q- O8 N6 ^
Another animation of PCR
) L; J$ C Y0 T# l+ Z2 a! f- ehttp://www.people.virginia.edu/~rjh9u/pcranim.html
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Animations of restriction digests and gel electrophoresis # E, k) G% y- e! I
http://www.biology.ucsc.edu/classes/bio20L/animate " _: A% l' ~# S Q
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Gel electrophoresis techniques and simulations ! I6 C: u/ x; P2 J6 `
A wonderful website that includes links to a virtual lab on agarose gel electr
( B+ y! U2 q9 _- v! Fophoresis: 9 F1 O- y5 t( `% P- u
http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/gels/ ( Y1 W* M' l6 S2 k
A Massachusetts Institute of Technology website dealing with Southerns, Northe
h2 G/ J# d3 b2 R vrns, Westerns, and cloning:
9 ^, b( H0 X1 L+ b% Ahttp://web.mit.edu/esgbio/www/rdna/rdna.html
8 K8 E" ]0 W% N8 n7 `. I2-D gel electrophoresis of proteins: " b- l* P$ N% t. o
http://www.fitzscipress.com/Chap05.pdf # E- P9 ]- A" W( f. w
A website containing lots of links to electrophoresis techniques and simulatio1 L( W# M2 }" e! J9 e z. B
ns:
. z" R0 }. j* J9 Z3 V: ohttp://biobenchelper.hypermart.net/pr/electrophoresis.htm 7 p0 [! M A% ^
Want to learn more about chemical bonds? Check out this website: " u, w7 q" A" ~3 t- j) L, h% H4 O) D$ E) S
http://hyperphysics.phy-astr.gsu.edu/hbase/chemical/bond.html
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多个录象-多个著名公司 % ~- P# `% h* u4 t) J+ ^" a* Z
http://www.biocompare.com/video.asp
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9 b N- k, {8 b" [+ u& e4 \5 R+ fPCR和 RFLP动画片 0 x. Q; [9 K6 m
http://medtech.cls.msu.edu/ISL/immunology/pcr.htm , K" Y# S; L! H3 e
http://medtech.cls.msu.edu/ISL/immunology/rflp.htm
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6 P6 m7 z9 K% { Jredasoft visual cloning 2000 patch ; M4 \3 _2 t4 O$ }
http://www.dxy.cn/bbs/post/view?bid=67&id=496454&sty=1&tpg=26&age=0 5 {, t, u5 X2 A# t( v% o
% q! U5 {. J: V8 t- H% r9 ^分子生物学屏幕保护程序下载
6 O" ^& u1 U l) \/ \, h5 apcr
w) \% B- @ K4 p c" R# ~http://biosoft.biosino.org/down/PCR.exe [8 z, w# A8 P6 F
蛋白 + x* E- a0 R6 C. Y% \- {
http://biosoft.biosion.org/down/ssaver.zip
4 P+ d* @3 L5 d' D/ \amavi100 , s+ {8 V6 I1 [5 E$ ]* g
http://ourworld.compuserve.com/homepages/esmsoftware/amavi100.zip & I1 N5 U3 I3 p6 } m) `$ i
dna旋转
1 ~/ S5 P% X/ s7 M- qhttp://www.rocketdownload.com/dnload/dna.exe / n6 F4 \1 R+ p$ ]# S
ddnabase
/ ?6 o; B* N1 }& ]5 c8 o fhttp://www.rocketdownload.com/dnload/ddnabase.exe
- I9 i) _5 F1 {cyclovir 6 b7 t$ h4 B" C" x
http://mama.indstate.edu/pfaffle/software/cyclovir.exe
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1 w) n9 E9 v# |' K! Vhttp://biosoft.biosino.org/down/source.exe 6 Y( @2 r$ m h$ Z
http://ftp.monash.edu.au//pub/win.galttech/gtmolecules.zip |
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