一个PCR相关的专业网站，内容很全 + K- _6 ?+ e5 o2 C. P3 d, s |0 ]4 a) @
http://www.pcrlinks.com/
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, h% @9 F3 Z; i, b. ~$ I$ ]! z/ v实验方法与步骤详解 H. X3 }6 I: ^' ~ a
http://swjsx.ntmc.edu.cn/Protocol/home.htm
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7 f( p2 {8 t7 e. ]: k$ Z美国AXYgen公司
: a$ T, g; d. O9 ~http://www.axygen.com/NewFiles/pcr.html
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3 b/ F D5 J- _http://www.alkami.com/
: ~6 D V& c9 `7 }* iThis 158 page PCR manual covers the following topics 4 a3 w" K) S* d/ T
PCR Primer design
9 N: |9 a" I5 h) `5 \3 FPCR Methods 5 _; H) y; C/ l( X0 K
PCR Polymerases
* V" C; H+ b0 k z( X1 ?7 OPCR Variables
: e) `1 w4 w; k2 P* h/ V% OPCR Troubleshooting
9 N2 z2 o) h2 YSpecial PCR Topics 0 }! Z; E- U3 o9 u1 l' R
Appendixes and a comprehensive Index
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& N5 A* |" s* B7 L7 b1 ?- IBioGuide - PCR x. P6 P0 H6 X
http://bip.weizmann.ac.il/mb/bioguide/pcr/contents.html : P7 m9 H" `$ b9 M+ z
魏茨曼基因组和生物信息学研究所的一个网页，介绍PCR的初步知识。
- {: {7 X) k) n% T, w; X包括：
" d9 Y$ k& a; q* E; Y( ^What is PCR? ' I7 L+ m4 U( \: R
What are some good reference books for PCR?
% {8 K) f9 n! \How should I select a set of primers to use for PCR? 1 |( I: O2 s5 ]# v |3 @
Programs for designing PCR primers
: S9 m# B0 |' e# Z' L `! ]What is "Hot-start" PCR?
! H7 p5 ?" Z6 o2 WWhat is AP-PCR or RAPD PCR? : F) d2 _- Z* l
What is "Touchdown" PCR?
2 s; D7 t; t, _( ]( u8 eIs there a simple method to sequence lambda, M13, or plasmid clones using PCR?
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i* T* l" Y w; q; JA Look at PCR Cloning Tools e: f8 P: S9 F% j y
http://www.dxy.cn/bbs/post/view?bid=67&id=151321&sty=1&tpg=63&age=0 - j( d% Z" b" t g# M
; O2 X: n# c; G0 R! N% c' N$ e! Y9 @PCR常见问题及回答 + [4 m6 s. e3 }, H% I
http://www.invitrogen.com.cn/techtitle/main/faq_PCR.htm#2
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; L+ w! I- R! hPCR和Realtime PCR的原理讲的很透, 一个介绍Realtime PCR的教学网站，里面的powerpo- l! B1 q8 Y3 A: {% h8 z& ~% l
int文件非常好 ' q8 j/ N% S8 \6 f2 ]# I+ a# L
http://www.med.sc.edu:85/pcr/realtime-home.htm
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SYBR Green PCR
2 |. Q7 J, [7 c0 s7 t" |; Q2 ~http://www.dxy.cn/bbs/post/view?bid=67&id=638781&sty=1&tpg=5&ppg=1&age=0#63878
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PCR QUICK GUIDE - R# p5 x# p" J/ @* L
http://www.wzw.tum.de/gene-quantification/quick-guide-pcr.pdf
: [. N/ _" J S$ H; R7 k9 |http://www.sfb363.uni-halle.de/mod-path/differential_display/sturtevant00.pdf
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0 p% t6 t- o5 qhttp://www.wooster.edu/biology/courses/bio309/default.html ) F( b5 j1 N$ n9 l
& W# R! _2 _8 _( ~5 O; i7 LPCR拼接
& p3 K5 X! j' Q5 Y' Z! }1 Ohttp://www.dxy.cn/bbs/post/view?bid=67&id=305337&sty=1&tpg=47&age=0
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知道蛋白如何寻找其氨基酸序列
' M* I* n. i% R' J/ }http://www.dxy.cn/bbs/post/view?bid=67&id=401195&sty=1&tpg=36&age=0
2 A/ \9 _; \* n [http://us.expasy.org/cgi-bin/niceprot.pl?P08253 ; b# P: M9 E! L& u: x1 G* Q! A2 P6 C
0 g' w& X H" S L$ ? {) ~1 |关于DNA的flash版和文字版
$ D, T5 h* [: X6 P) chttp://www.pbs.org/wgbh/nova/genome/dna.html#
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ABI Prism 7000 简要使用说明 % j. Y% N. V2 J- ~0 _8 x/ z8 x" O8 q( t
http://www.genomultix.com/tech/7000.htm 9 ]8 O7 J, Y0 d4 I: z( Q
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好书推荐：Practical Protocols of Gene Engineering
" H K& q j" s" ]5 \: L( ahttp://www.estarray.org/books/ 2 I* Y: p; n+ t ?9 t2 ?6 c
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http://www.life.uiuc.edu/bio100/lectures/s02lects/18s02biotechclonerecomb.html" A6 j+ l. d5 U/ H' ^
7 H$ Z5 c3 m; aWebsite dealing with cloning and molecular analysis of genes
3 T! d$ C8 A0 G; i$ u3 Ahttp://www.ndsu.nodak.edu/instruct/mcclean/plsc431/cloning/clone3.htm
# c# p) H4 u8 _ F5 ?5 p7 H1 X% TSome really wonderful illustrations of the techniques of genetic engineering! 1 q0 Q7 `; f0 R! F4 a7 u# \
+ L. _! d2 x: `; y6 Vhttp://www.uic.edu/classes/phar/phar331/lecture6/ 3 _1 `% @$ Q# n$ H
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A truly amazing global resource page on cloning, stem cell research, and relat
5 s; W" V2 n# Y! G# w2 |ed ethical issues, including recent news articles and legislative updates ( y, y* r) n+ ]
http://genomics.phrma.org/cloning.html 9 W, j) G& q. D) B6 |) K5 y, ?
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Animation and article on interspecies cloning . A& G% W5 Q" X( G3 o
http://www.brookscole.com/chemistry_d/templates/student_resources/0030244269_c) Y+ \1 ` s7 W6 N# i
ampbell/HotTopics/InterspeciesCloning.html
0 w) J8 ], D' IFrom PBS...an animation that illustrates how stem cells are cultivated ( |2 N. b1 v+ |' O$ ` n
http://www.pbs.org/newshour/health/stem_cells.swf
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7 ]. N6 A" {$ V/ S! N; tAnimations illustrating the use of viruses as vectors for gene therapy % a- y0 U3 f; @9 C
http://www.technologyreview.com/articles/5940.htm
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Animations of techniques used for cloning mammals, as well as a create-your-ow
5 D2 R& S) T1 m" e, F8 f3 y* nn-clone link
# X+ s9 y- O8 ^6 ^, q$ [% |" ~http://library.thinkquest.org/24355/data/details/media/index.html 7 W, [0 G6 Q0 \
Another animation of PCR
, R& G, ^8 e+ ?# z! _4 yhttp://www.people.virginia.edu/~rjh9u/pcranim.html
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7 [9 a2 {7 T2 O5 R/ ]2 aAnimations of restriction digests and gel electrophoresis
' m; G2 k# K4 @5 Q2 j7 o* h" ahttp://www.biology.ucsc.edu/classes/bio20L/animate 6 a0 \- }! e' e3 v& W$ ]" P: u( j
( a3 l' v* ]) i% ]4 G/ KGel electrophoresis techniques and simulations 4 w" t/ x. X. E3 f- U4 Q+ y% o, s
A wonderful website that includes links to a virtual lab on agarose gel electr* C. r# ^+ @! a9 A, Z: A8 X: [
ophoresis: / @0 N+ y+ ]2 y! Y) y4 J
http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/gels/
" n1 S9 N' b3 W' e+ m& F9 kA Massachusetts Institute of Technology website dealing with Southerns, Northe
+ j0 G, V, d2 |/ K& j/ O, S8 Q! urns, Westerns, and cloning: 9 R/ o$ q# X) s* v5 v
http://web.mit.edu/esgbio/www/rdna/rdna.html 9 ?% c3 k' [% @5 `, h0 T& ?
2-D gel electrophoresis of proteins: ( v/ @ Q6 a& R Y# C6 ]# q
http://www.fitzscipress.com/Chap05.pdf / J$ f8 m- v& l$ a1 [ T
A website containing lots of links to electrophoresis techniques and simulatio
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http://biobenchelper.hypermart.net/pr/electrophoresis.htm
! w. {: f" o. E6 D! }Want to learn more about chemical bonds? Check out this website:
/ d. L$ O X& G+ Fhttp://hyperphysics.phy-astr.gsu.edu/hbase/chemical/bond.html
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多个录象-多个著名公司
, z& N7 \" H; t7 T. h! thttp://www.biocompare.com/video.asp
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PCR和 RFLP动画片
# K! ~3 {( l4 B: I) b5 }http://medtech.cls.msu.edu/ISL/immunology/pcr.htm
" w( l0 t" T6 q( X. O( ihttp://medtech.cls.msu.edu/ISL/immunology/rflp.htm : v/ d$ y- r9 \# J" S
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redasoft visual cloning 2000 patch 8 h' {" c- s+ f
http://www.dxy.cn/bbs/post/view?bid=67&id=496454&sty=1&tpg=26&age=0 % d, }. ` G1 e
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分子生物学屏幕保护程序下载
6 _) @& F6 H* x2 upcr
Y9 Y; b+ O' khttp://biosoft.biosino.org/down/PCR.exe
% C* d& E: L2 R, w6 `. k2 b蛋白
( F! A/ S& c; `% Z+ _! z i' Ohttp://biosoft.biosion.org/down/ssaver.zip ; b m+ f$ c; I: T1 h
amavi100 / u$ D* ]& u4 z, f5 b2 L0 Y* W+ F
http://ourworld.compuserve.com/homepages/esmsoftware/amavi100.zip
" P; ^7 @" W! H5 m. B( C' E- V8 Adna旋转 0 V/ L5 D4 @. R0 n3 ^2 l, ?9 B
http://www.rocketdownload.com/dnload/dna.exe
/ m# ^1 p) {4 K1 `3 Wddnabase 7 c j4 {! j8 S+ |1 j' M: j
http://www.rocketdownload.com/dnload/ddnabase.exe
, k% x! b7 T7 \+ q8 \cyclovir ; C, b' c* ^# O
http://mama.indstate.edu/pfaffle/software/cyclovir.exe
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http://biosoft.biosino.org/down/source.exe
( U! J+ |$ n2 y5 Z3 @. Ihttp://ftp.monash.edu.au//pub/win.galttech/gtmolecules.zip |
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